Tuberculosis is one of the world’s leading bacterial infections caused by Mycobacterium tuberculosis (M. tuberculosis). It is contracted through the air and targets the lungs, and if left untreated, the disease may be fatal. With over 2.5 million cases just in Central Africa and over 400 thousand deaths each year, it is the second leading cause of death in the region. In this predominantly rural area, the primary identification method for this disease is a molecular diagnostic test called GeneXpert, which requires several hours to run, exacerbating the disease’s progression. The design explored in this paper–TB-EZ–modified a traditional lateral flow assay to create a rapid antigen test that utilized capillary action. With this test, the goal was to detect the cell wall protein of M. tuberculosis, Ag85, as the antigen. This was achieved by designing a buffer that would lyse the M. tuberculosis bacteria so that the Ag85 antigen could be released for the antibody to bind to. The first antibody, EPR28401-54, would bind to gold nanoparticles and rest on a nitrocellulose membrane, which is porous and nonpolar to help facilitate capillary flow. Unsweetened milk powder was used as a surfactant for the nitrocellulose membrane to prevent protein complexes from binding to the membrane at undesired locations. As the antibody-gold complex traveled to the detection line, it would bind with another antibody, and the gold nanoparticles would be released. This then produces a color for visual identification. Through further research, interactions between the antibody, surfactants, blocking agents, and lysis buffer will be tested for more precise results. This test has the potential to affordably and efficiently reduce deaths caused by the pathogen through the rapid detection of M. tuberculosis to allow for further action to be taken immediately.