Overfishing continues to affect fish populations, especially for populations affected by pollution or other outside factors, that would not be able to recover. So, our group sought ways to make a portable, affordable, and accurate test for stress hormones or signs of reproductive harm in fish populations. With this test, at risk populations could be identified with greater detail and convenience. Our research led us to a couple ways to design the test, an ELISA test, and a color gradient test. Ideally, the test would be able to show the gradient of the result, and would be cheap and convenient.