Shinorine, a UV-absorbing compound, is synthesized from sedoheptulose 7-phosphate via for key genes, with Ava_3855 facilitating its final conversion. To express it in E. coli BL21(DE3), genes are inserted into a plasmid (e.g., pET18) using Golden Gate or Gibson Assembly and induced via the T7 promoter. Co-expression with Scytonemin enhances UV protection. This bioengineered alternative to chemical sunscreens offers a sustainable, water-soluble solution while addressing regulatory and ethical concerns.