Back to Project Library

Radon is a toxic, radioactive gas that is produced from the decay of uranium, often found in soil. Radon is harmless at low concentrations. However, when radon becomes trapped inside of an enclosed space, it accumulates and results in a dangerously high concentration of the gas. This is most often found in buildings where radon seeps inside through breaches in foundations. When exposed to high concentrations of radon for 2-3 years, humans can develop health issues such as shortness of breath, chest pains, fatigue, and coughing up blood. Long term exposure of 5-25 years even causes cancer. According to the American Cancer Society, radon is responsible for about 21,000 lung cancer deaths each year in the United States. As radon is colorless, tasteless, and odorless, it is often forgotten and goes untested. Moreover, there aren’t any specific medical tests that can assess individual exposure to radon gas. This invisibility makes radon extremely dangerous. While radon test kits are available, many homeowners forget to purchase and use them regularly. Our project involves genetically altering the houseplant Tradescantia, commonly known as spiderwort, to change color when exposed to high concentrations of radon—effectively alerting those around to evacuate and treat the high concentration of radon appropriately. Tradescantia will be used because its DNA has been proven to be damaged by radon exposure. The proposed design adds a specific operator sequence operator site called a Cheo box to the promoter region of the recA promoter. The recA promoter is activated by DNA damage caused by radiation such as radon. It has been proven that the addition of an extra Cheo box in the promoter region of recA will increase the responsiveness to radiation. We will insert this altered promoter into a plasmid that contains an orange fluorescent protein (OFP) gene. This fluorescent protein will be triggered by the recA promoter, causing the plant to glow orange when presented with radon-induced DNA damage and thus fulfilling the purpose of warning the houseplant owner of high radon levels by producing a visible signal. Adding superoxide dismutase, an enzyme capable of breaking down reactive oxygen radicals and lowering the O2¯ level within an organism’s cells, after the fluorescent gene will allow the plant to absorb and break down the radiation. Under this same recA promoter, the superoxide dismutase will effectively begin to break down the radioactive particles within Tradescantia’s cells.

Fluorescent Flora: Detecting Radiation with Plants

School

Western Reserve Academy
Hudson, OH

BioBuilderClub Season

2021-2022 Season

Category

Resources

Detecting high levels of radiation with the fwYellow pigment in engineered spiderwort_Western Reserve Academy BBC_21-22

2022_BBC Lightning Talk_fluorescent flora

2022_BBC Poster_Fluorescent Flora