Polyfluoroalkyl substances (PFAS) are commonly found in plastics, clothing, adhesives, and food packaging. These chemicals accumulate over time in humans and animals, leading to serious health risks, including an increased risk of cancer, thyroid disease, liver damage, immune system dysfunction, and developmental issues. Furthermore, they exacerbate environmental issues by contaminating soil and ecosystems near water sources. Unfortunately, PFAS are considered “forever chemicals” due to their robust carbon-fluorine (C-F) bonds, causing them to resist natural degradation. This project addresses the growing issue of PFAS through degradation and offers a safe solution to benefit environmental and human health. The project design involves modifying Pseudomonas putida as a chassis due to its ability to degrade many natural compounds and its resilience in various environments. The production of modified chemoreceptors enables binding with PFAS around the cell, triggering flagellar movement. Movement occurs toward higher concentrations of PFAS, similar to microbial chemotaxis toward nutrients. Concurrently, the prmA promoter activates enzyme production. The secretion of the 4-fluorobenzoate dehalogenase enzyme facilitates the degradation of PFAS in water through defluorination reactions. The next steps are to fully implement modified DNA into the chassis and test for core functionality in different environments and types of PFAS. In conclusion, the project aims to engineer P. putida with modified chemoreceptors and a prmA-regulated enzyme system to detect and degrade PFAS in water, managing the growing risks that PFAS pose.