Plastic pollution is a crucial environmental issue due to the human incapability in preventing plastic waste. Currently, solutions to this problem include using enzymes to degrade plastic contents and replacing single-use plastic with biodegradable plastic; however, these methods are time consuming and costly to be performed on a mass scale. Our project aims to synthesize polyhydroxyalkanoates (PHA), which is produced in Bacillus subtilis, to mass produce biodegradable plastic. PHA is a type of bioplastic that can substitute a variety of today’s single-use plastics due to having elastomeric properties. Therefore, we want to increase the efficiency and accessibility of PHA production by isolating the genetic part that synthesizes PHA in Bacillus subtilis and transform that part into E.coli to maximize the production of PHA. The extraction of PHA from the bacterial cells will be facilitated through a lyophilization, dehydration, and centrifugation process. Ultimately, we hope to minimize plastic pollution by designing a genetic system in E.coli that efficiently produces a more biodegradable plastic, PHA.