This project investigates the impact of varying concentrations of euglena powder prebiotics on Lactobacillus growth, alone and with E. coli K12. Previous studies show euglena powder stimulates Lactobacillus growth, but its effect on Lactobacillus-E. coli co-cultures is unknown. Lactobacillus plays a key role in lactose breakdown, producing lactic acid that can cause intolerance symptoms. Our study aims to assess euglena powder’s efficacy in promoting Lactobacillus growth, potentially aiding lactose intolerance management.