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Memphis is home to many warehouses and energy plants. On the surface it may not seem like a big deal but when you start to notice citizens in certain areas developing higher incidence of cancer you start to wonder why and investigate. The location of sterilization facilities that use Ethylene Oxide in poor communities is a complex issue that involves multiple factors, including zoning regulations, political power, economic factors, and historical patterns of discrimination. A historical poor community in Memphis called South Memphis, is coming to terms with this reality. Local citizens are dealing with the effects of EO which is an organic compound that is often used in antifreeze and used in sterilization processes. EO can be very damaging to one’s DNA and can even cause cancer. EO is a highly reactive and toxic gas that can damage cells through a variety of mechanisms. It is a powerful alkylating agent that can react with cellular macromolecules such as DNA, RNA, and proteins, leading to cellular dysfunction and death. Our team will create a windmill that will detect EO and break it down. Using staphylococcus saprophyticus, a strain of bacteria used in curing meats and cheese, to uptake a plasmid that has been modified to produce green fluorescent protein (GFP) will be inserted through transformation, will allow for a safe living organism that can be used as a monitor that can be easily tracked. A GFP detection device can then be created to indicate when the bacteria stops glowing as it comes in contact with EO due to cellular damage and death that reduces the amount of GFP being expressed. A device that is able to circulate in the air will allow for small amounts of EO to be detected as the device moves.

Addressing Ethylene Oxide in Soil and Air

School

Crosstown High
Memphis, TN

BioBuilderClub Season

2022-2023 Season

Category

Resources

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