Hematopoietic stem cells (HSCs) are essential for bone marrow transplantation but are rare and difficult to expand in vitro due to their tendency to differentiate. As a result, patients with leukemia and other blood disorders are forced to rely on donor bone marrow transplants, which are limited by low stem cell counts, immune rejection, and invasive procedures, often requiring repeated low-dose infusions which are painful for both donors and patients. Our project proposes a bioengineered platform that models the HSC niche to preserve and expand HSCs before transplantation. By mimicking paracrine signaling to maintain stemness, our approach could generate transplant-ready HSCs from patient-derived iPSCs, reducing donor dependence, minimizing immune complications, and improving transplant outcomes.