Our biosensor demonstrates the potential to significantly reduce the cost and increase accessibility of DHT testing. Traditionally, DHT levels are measured using High-Performance Liquid Chromatography (HPLC), a method that is expensive, time-consuming, and inaccessible to many. Our study introduces a more affordable, rapid, and accessible alternative using a synthetic biology approach. We have developed a design of a biosensor employing Transcription Factors (TFs) from Pimelobacter simplex, known for their responsiveness to hormones.